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A study led by researchers at Macquarie University in Australia revealed a new way to test whether a person is taking human growth hormone (GH) by testing protein levels. Specifically, researchers use proteomics to discover which protein levels change when a person takes recombinant human growth hormone. Related articles were published on Scientific Reports on August 30th.
The peptide hormones in the Doping List issued by the World Anti-Doping Agency (WADA) contain human growth hormone. Human growth hormone is produced by the secretion of pituitary gland. The most important physiological function is to induce hepatocytes to produce insulin-like growth factor; promote the division and proliferation of cartilage, bone, muscle and other tissue cells; promote the growth and metabolism of the body, increase the size and weight of the muscles. . As a new type of stimulant, peptide hormones have certain difficulties in detection, and their abuse has a significant upward trend in the world.
Detection of new target proteins in the blood
Alamgir Khan, the author of the article, said: "We were able to detect changes in the levels of a group of eight proteins found in human plasma when a person took recombinant human growth hormone. Six of these proteins were previously not affected by human growth hormone. Found in the study."
He added: "We found two proteins, APOL1 and fetuin-A, which provide evidence of active hormones, while another protein, called vitamin D-binding protein, may be useful because its levels are reduced by the use of hormones. And often stay at a low level for a longer period of time. This means that anti-doping officials will have a longer time to discover athletes taking hormones."
Improved the difficulty of detecting recombinant human growth hormone
The concentration of recombinant human growth hormone in the blood is ng / L level, the half-life in the blood after intravenous injection is only about 15 ~ 20 m in, after the subcutaneous or intramuscular injection in the blood concentration reached 1 ~ 3 h The peak value was not detected in the circulating blood after 48 h.
Two current anti-doping agencies for detecting recombinant human growth hormone include a test to determine changes in the ratio of artificially and naturally occurring growth factor proteins, and another test to detect an increase in the levels of both molecules after taking the hormone.
Dr. Khan explained: "The problem with these tests is that they need to be completed shortly after taking the hormone, and the test results vary from person to person. Our findings identify other blood markers that may help to replenish Existing biomarker testing provides more confidence to anti-doping authorities, athletes and the public, allowing them to enjoy a purer sport."
This method can also be used for the detection of other hormones.
Recombinant human growth hormone stimulates cell growth and production. It was originally licensed to people with growth hormone deficiency, but was abused by some athletes to become one of the drugs that improve performance. The researchers said their findings also heralded the development of more powerful and sensitive detection methods for testing a range of artificial body enhancers.
Dr. Khan concluded: "The proteomics used in this study can also be applied to other body function enhancers."
Another author, Director Mark Molloy, concluded: "The key to the results is the use of proteomic analysis of gel technology and advanced mass spectrometry, coupled with well-designed clinical research. Research shows that in promoting purification campaigns, high quality The effectiveness of proteomic analysis."
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