Analysis and Application of Berberine Hydrochloride by HPLC

Berberine hydrochloride:

P / N: Chenhang chromatography column 88182545 C18, 5u 250 × 4.6mm

Chromatographic conditions:

Mobile phase: Hydrochloride buffer [0.05mol / L KH2PO4 and 0.05mol / L sodium heptane sulfonate (1: 1), containing 0.2% triethylamine and adjusted to pH = 3.0 with H3PO4]: Acetonitrile = 60: 40

Detection wavelength: 240nm Flow rate: 1.0ml / min