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The principle and classification of ELISA 1. The principle of ELISA The basis of ELISA is the solidification of antigen or antigen and enzyme labeling of antigen or. The antigen bound on the surface of the solid phase carrier may still maintain its immunological activity, and the enzyme-labeled antigen or antibody retains both its immunological activity and enzyme activity. During the measurement, the test specimen (the antibody or antigen in it) is reacted with the antigen or antibody on the surface of the solid phase carrier. The antigen-antibody complex formed on the solid phase carrier is separated from other substances in the liquid by washing. Enzyme-labeled antigens or antibodies are added, and are also bound to the solid-phase carrier through the reaction. At this time, the amount of enzyme on the solid phase is proportional to the amount of test substance in the specimen. After adding the substrate for enzyme reaction, the substrate
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