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The Human Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4) ELISA Kit determines the concentration of the standard by plotting it on the y-axis (logarithmic scale), while the corresponding OD values are placed on the x-axis (also logarithmic). A standard curve is then created on log-log paper. It is recommended to use specialized software, such as CurveExpert 1.3, to generate the curve and perform accurate analysis. Once the standard curve is established, the sample's OD value can be used to determine its corresponding concentration from the curve, and then multiplied by the dilution factor to obtain the actual sample concentration. Alternatively, you can calculate the regression equation of the standard curve using the known concentrations and their respective OD values. By substituting the sample’s OD value into this equation, you can directly compute the sample concentration and then apply the dilution factor to get the true concentration. This method ensures high accuracy and reliability in quantifying LILRB4 levels in biological samples. Always follow the manufacturer's instructions and validate results with appropriate controls.
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