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The Human Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4) ELISA Kit determines the concentration of the standard by plotting it on the y-axis (log scale), while the OD value is plotted on the x-axis (also log scale). A standard curve is then created using logarithmic graph paper. It is recommended to use specialized software, such as CurveExpert 1.3, for accurate curve fitting and analysis. Once the standard curve is established, the sample's OD value can be used to find the corresponding concentration from the curve, and then multiplied by the dilution factor to obtain the actual sample concentration. Alternatively, you can calculate the regression equation of the standard curve based on the known concentrations and their corresponding OD values. Then, input the sample's OD value into this equation to calculate the concentration, and again multiply by the dilution factor to determine the true concentration of the sample in the original solution. This method ensures accuracy and reliability in quantifying LILRB4 levels in biological samples.
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