Issues needing attention on digital camera microscope photography
The use of digital cameras for microscope photography has now become a very effective means. Convenient and quick imaging; preview, save, delete or print on the spot has made new developments in microphotography.
At present, when using a digital camera to take microscopic images, most of them use digital digital cameras (such as NIKON 4500, CANON G6, A620, etc.), which is a camera whose camera lens is not removable. In this way, an intermediate lens needs to be added between the microscope and the camera lens, so that the microscopic image is on the focal plane of the camera-we call it the relay lens.
The quality of this lens directly affects the quality of the captured microscopic image. Since there is currently no unified inspection standard for relay mirrors, and there is no consensus between users and producers, it is difficult to distinguish good from bad. Therefore, many manufacturers have appeared, and the relay mirrors on the market are mixed, and the quality is uneven. Based on our years of work in microscopes and our understanding of digital photography, and comparing the pictures taken by various relay lenses at home and abroad, we have summarized several methods for identifying the advantages and disadvantages of relay lenses for your reference:
One. Image size
It is generally believed that the relay lens is added as long as the image can fill the camera
The LCD screen is successful, it is a qualified relay mirror. It is only a minimum standard for the relay lens to fill the screen, and some have to use up the optical zoom of the camera to make the screen full. The optical system of such a relay lens is defective.
two. Image color reproduction
The reproducibility of the color of the photographed microscopic image is also a standard for testing relay lenses. A qualified relay lens requires that the color of the image taken is consistent with the color of the image observed by the microscope.
three. Depth of field
Anyone who knows photography knows that shooting an image requires a certain depth of field (except for art photos). We all have a certain sense of hierarchy when observing microscopic images. A qualified relay lens should have a depth of field not less than the microscope objective lens.
four. Image sharpness
This is the most important index for testing relay mirrors. The so-called "sharpness" is the resolution we usually say, the level of this index directly affects the quality of the captured microscopic image.
In short, the ultimate criterion for identifying a relay lens is to ensure that the quality of the image taken with the digital camera and the image observed in the microscope are as consistent as possible.
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