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Ultraviolet detector is a commonly used detection instrument, which is mainly used for quantitative analysis of ultraviolet absorption samples in agricultural research, chemical, food, medical and other units, and is widely used in many fields. Today, Xiaobian mainly introduces the working principle and application of the UV detector, hoping to help users better apply the product.
How the UV detector works
The basis of the working principle of the instrument is the law of light absorption. The light emitted from the light source passes through the slit, the filter, and the sample cell to the photo-electric tube, so that the change of the light intensity caused by the difference of the sample concentration is converted into a change of the photocurrent, and the photocurrent is input to the logarithm through the amplifier. The converter converts the transmittance T into an A output, that is, A=1g — = εCL where ε is the molecular extinction system of the sample to be tested, C is the sample concentration, and the unit is gram/liter unit, and L is the optical path. Use centimeters as the unit. According to the above formula, it is known that A is measured, and the sample concentration C is known. If the recorder is directly input from the amplifier, the map of the change in the transmittance T of the sample is plotted. If the input from the logarithmic converter is input to the recorder, the spectrum of the change in the optical density A of the sample is plotted.
UV detector use
1. In scientific experiments, it is to detect many major substances such as proteins, nucleotides and so on.
2. In the production and research of drugs, it can be used to check the quality of various kinds of fluorescent drugs such as hormone alkaloids and vitamins. It is especially suitable for thin layer analysis, paper layer analysis and detection.
3. In the chemical industry such as dye coating rubber and petroleum, various fluorescent materials, fluorescent indicators and additives are determined to identify different types of crude oil and rubber products.
4. It can be used in textile chemical fiber to measure different kinds of raw materials such as wool, silk rayon, cotton synthetic fiber, and check the quality of finished products.
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