Basic principles and application guidance of chemiluminescence

The basic principle of chemiluminescence (Chemiluminescense) is the chemical reaction between the two substances A and B to generate substance C. The energy released by the reaction is absorbed by the molecules of substance C and transitions to the excited state C *. The process of excited C * returning to the ground state Produces optical radiation. Due to the phenomenon of light radiation accompanying the chemical reaction, it is called chemiluminescence. It is often used to detect proteins and DNA in the field of biology. It does not require an excitation light source such as ultraviolet light during the reaction. It relies on specific enzymes such as HRP or AP to bind to the substrate to promote the reaction. The optical radiation usually generated is very weak, and the optical signal is not easy to collect. Even with continuous exposure to the reaction process, due to poor imaging quality, accurate quantitative analysis cannot be performed.
Now, with the continuous improvement of CCD imaging technology, the sensitivity to weak signals is greatly improved, so quantitative analysis of chemiluminescence results is no longer a difficult task. The Fusion imaging system produced by the French company VILBER can provide the best exposure pictures for chemiluminescence users. Combined with the powerful analysis function of Bio-1D software, it can provide users with the most accurate quantitative analysis.
Application display 1. ELC Western blot
Sample: Mouse immunoglobulin labeled with peroxidase secondary antibody Method: Western blot / PVDF Membrane Substrate: ECL
Exposure time: 5 minutes Instrument: Fusion FX5

2. CDP Star—Dot blot
Sample: Digoxin-labeled DNA probe hybridized with Fab fragment Sample method: Dot blotting / PVDF membrane Substrate: CDP Star
Exposure time: 1 minute Instrument: Fusion FX7

3. Luciferase assay
Sample: Luciferase detection method: Petri dish
Substrate: Fluorescein Exposure time: 3 minutes Instrument: Fusion FX5

Flame Retardant Nonwovens Fabric

HUZHOU JIHAO NONWOVENS FABRIC CO.,LTD , https://www.jihaononwovens.com

Posted on