Classification of immunoenzyme technology

The classification of immunoenzyme technology has not been unified. Some scholars start from the detection of antigen or antibody in the solid phase or the liquid phase, and divide the immunoenzyme technology into liquid phase immunoenzyme technology and solid phase immunoenzyme technology. Solid-phase immunoenzyme technology is divided into enzyme-linked immunosorbent assay (ELISA) and limited antigen substrate bead system assay. Later, dot-ELISA, ELISA and dot-ELISA were developed in the method of detecting antigens or antibodies. The main difference is that the solid phase carrier used is different. The carrier in the ELISA system usually uses polystyrene material, and Dot-ELISA usually uses white nitrocellulose film. Since the cellulose membrane has a strong adsorption effect on antigens or antibodies, the detection sensitivity is improved, and the detection time can be shortened. At present, dot-ELISA is becoming more and more widely used, especially in the screening and isolation of monoclonal antibodies and purification of monoclonal antibody hybridoma cell lines. Some scholars separated the bound and free enzyme markers after the test antigen (or antibody) reacted with the enzyme-labeled antibody (or antigen) and then carried out the measurement and classification, and divided the immunoenzyme technology into homogeneous (Heterogene -ous) immune enzyme technology and homogeneous (Homogeneous) immune enzyme technology. The so-called heterogeneous immunoenzyme technology is to separate the bound or free enzyme markers, and then measure the enzyme activity to show the amount of antigen (or antibody) measured. It includes most types of immunoenzyme technology. The so-called homogeneous immunoenzyme technology does not need to separate the free and bound enzyme labels, and directly binds the enzyme labels to the antibodies, and then measures according to changes in enzyme activity. This method is more complicated to use, and the detection results are more difficult to be consistent, so it is not widely used, and there are few reports.

The above article was collated and published by Shanghai Yanjin Biotechnology Co., Ltd. Shanghai Yanjin Biotechnology Co., Ltd. specializes in selling ELISA kits of various brands. Serving universities and immunology research units. Quality assurance and perfect after-sales service. And can be free to detect, better service for you.

Cookware Sets

Pots And Pans Set,Best Cookware,Best Cookware Set,Nonstick Cookware Set

GUANGDONG INWIN INDUSTRIAL GROUP CO. ,LTD. , https://www.inwin1979.com