Corticotropin-releasing hormone release kit instructions CRH RIA KIT

Corticotropin-Releasing Hormone (CRH) Release Kit Instructions – CRH RIA Kit Corticotropin-releasing hormone (CRH), also known as corticotropin-releasing factor, is a 41-amino acid peptide primarily produced by the paraventricular nucleus of the hypothalamus. It plays a crucial role in regulating the release of adrenocorticotropic hormone (ACTH) from the anterior pituitary gland, which in turn stimulates the adrenal cortex to produce cortisol. In addition to its central function, CRH is also expressed in peripheral tissues such as the placenta, thymus, spleen, and even activated T lymphocytes, highlighting its broader physiological significance. The CRH RIA kit provides a reliable method for quantifying CRH levels in biological samples. The kit includes six standard concentrations (0, 1.5, 5.0, 15.0, 45.0, and 135.0 ng/mL), along with labeled CRH (¹²⁵I-CRH), specific CRH antibody, buffer solution, and a separation reagent. These components work together to ensure accurate and reproducible results in radioimmunoassay procedures. Sample Preparation: For plasma collection: Draw 3 mL of venous blood into a tube containing 0.5 M EDTA-Na₂ (30 µL). Mix thoroughly, centrifuge at 3000 rpm for 10 minutes, and collect the plasma. Plasma samples can be stored at -20°C for up to 2–3 months without significant loss of activity. For tissue extraction: Take an appropriate amount of tissue, weigh it, and boil in 1 mL of saline for 3 minutes. Add 0.5 mL of 1N glacial acetic acid, homogenize, neutralize with 0.5 mL of 1N NaOH, and centrifuge at 3000 rpm for 30 minutes. Store the supernatant at -20°C for future use. Assay Procedure: 1. Prepare all reagents according to the instructions. 2. Add 200 µL of buffer to each well. 3. Add 100 µL of standards, control, or sample. 4. Add 100 µL of CRH antibody and 100 µL of ¹²⁵I-CRH. 5. Incubate at 4°C for 24 hours. 6. Add 500 µL of separation reagent and mix. 7. Centrifuge at room temperature for 15 minutes at 3500 rpm. 8. Remove the supernatant and measure the radioactivity in the pellet using a gamma counter. Data Analysis: Calculate the percentage of bound radioactivity using the formula: % B/B₀ = (B / B₀) × 100. Plot a standard curve on semi-logarithmic graph paper and determine the CRH concentration in unknown samples accordingly. Technical Specifications: - Sensitivity: ≤ 3 pg/mL - Intra-assay CV: < 10% - Inter-assay CV: < 15% Note: Each batch of the kit comes with specific dilution guidelines for standards, antibody, and labeled CRH. Always follow the provided instructions for optimal performance. For more information or to purchase this kit, visit Shanghai Xinfan Biotechnology Co., Ltd. or scan the QR code below to access the official purchasing platform. [QR Code Image] [Logo Image] Disclaimer: All content is protected by copyright. Unauthorized reproduction or use is prohibited. For inquiries, please contact us within two weeks of publication.

Women's Phone Bag

Cellphone Pouch,Mobile Phone Bag,Phone Bags For Women,Mobile Phone Bags For Women

NINGBO SUREWAY E-FASHION IMPORT AND EXPORT CO,.LTD , https://www.surewaybags.com