Effect of exogenous factors on the detection results of elisa kit

Effect of exogenous factors on the detection results of elisa kit

1 specimen dissolution m: due to various reasons, the specimen hemolysis, can release a large amount of m red peroxidase activity when dissolved by red blood cells, in the ELISA assay labeled with horseradish peroxidase, Can cause non-specific color development and interfere with the measurement result J. Therefore, the specimen must be collected to avoid dissolution.

. 2 specimens are contaminated by bacteria: the bacteria may contain endogenous horseradish peroxidase. Therefore, the specimen contaminated by bacteria can produce non-specific coloration and interfere with the measurement results.

3 specimens are improperly preserved: specimens that have been stored for too long in the refrigerator, 10} IgG can be polymerized into multimers, and AFP forms dimers, which can lead to excessive background and even false positives in indirect ELISA. The ELISA should be a fresh specimen. If it cannot be measured immediately, the serum samples measured within 5 days can be stored at 4~C. The specimens measured after 1 week should be cryopreserved; the specimens melted after cryopreservation, the protein is concentrated locally. The distribution is uneven, and should be mixed thoroughly before measurement.

4 specimen agglutination incomplete: m fibrinogen remains in the m clear, can form macroscopic fibrin blocks in the ELISA assay, easy to cause false positive results, so if using anticoagulation tube, should be fully mixed after blood collection; In order to prevent anticoagulation m, it should be fully solidified and then centrifuged for clear detection.

Crushing Machine, in a word, is a kind of machine to grind large size material or granule to smaller sizes or powder. Based on different request, Crushing machine can be divided into different types. Based on different material, different crushing machines are considered to meet different request.

For grinder large material into smaller sizes, we can use coarse crusher: This machine is a hammering type mill used to pulverize drugs, chemical materials, food staff and other materials with medium hardness and low viscosity. It is suitable for the suitable for the production in middle and small-batches or sample experiments in laboratories. Materials are fed into the pulverizing chamber by a helical conveyer, and pulverized instead of other energies of other energies of revolving parts of the machine. .
The machine is simple in structure and the pulverizing chamber can be dismounted assemble and be dismounted and cleaned easily. All the parts in the chamber which contact materials are treated specially and corrosion resistama. As a result, in addition Any material ,drifted into grind chamber through feed bucket ,punched by knife edges and knife stands ,cut and grinded simultaneously by revolving and fixed knives, and due to the centrifugal force of revolving object ,automatically runs to exit

If we grind small particles to powder: The crusher mill machine is widely used in pharmaceutical, food, chemical, it can crush various of material.

The machine is made up of feeding material part and crush part, and dust collect part. Its coniguration is very briefness, all the parts which contact the material are made of stainless steel.

Crushing Machine

Crushing Machine,Stone Crusher Machine,Mobile Crusher,Cone Crusher

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