![<?echo $_SERVER['SERVER_NAME'];?>](/template/twentyseventeen/skin/images/header.jpg)
Possible problems and solutions during the operation of rat connective tissue growth factor (CTGF) ELISA kit:
Why does the ELISA kit have a whiteboard and the positive control does not develop color?
1, no A, B color solution - do not miss the addition of liquid A and liquid B;
2, the preparation of the washing liquid is wrong - please prepare according to the dilution factor shown in the instruction manual;
3, no added enzymes and considered to have been added - be careful not to miss;
4. The stop solution is misused as a dilution of the washing solution or when the substrate is used - the label should be seen before each addition.
Why is the ELISA kit light in color and low in sensitivity?
1. The kit is too long in transit, the temperature is too high - try to shorten the transportation time, and the ice should be cooled in the summer;
2, the kit is not fully balanced - the kit is removed from the refrigerator at 2 ~ 8 ° C, open the lid, equilibrate at room temperature for at least 20 minutes, to ensure that all reagents have been equilibrated to room temperature (about 25 ° C);
3, the temperature of the incubator is less than 37 ° C - pay attention to the temperature of the incubator, after the reaction plate is placed, try to reduce the number of opening times to avoid affecting the temperature constant, especially for non-water-blocking incubators;
4. Insufficient holding time - correct timing of accurate clock;
5. The impact force during washing is too large, the soaking time is too long, and the number of washings is increased - the washing time is kept according to the instructions, and the washing times are accurately remembered;
6. The pipette has insufficient liquid absorption, too much water is hanged on the inner wall of the nozzle or the inner wall is not clean. Correct the pipette, the nozzle should be matched, the nozzle should be tight when it is installed, and the inner wall of the nozzle should be clean. use;
7. There is a problem with the quality of distilled water – use freshly qualified distilled water;
8, the substrate action time is insufficient - accurate timing.
Why is the ELISA kit reproducible?
1, the number of samples varies, the loading time is long and short - when repeating a sample, the loading time is as close as possible to the first time;
2, the holding time is inconsistent, the washing conditions are inconsistent, the operator is inconsistent - repeated determination of the specimen, operating conditions, personnel, etc. should be as consistent as possible with the previous time, in order to eliminate the possibility of inconsistency caused by these factors;
3. The sample loading is inconsistent - the sample should be thoroughly mixed before dilution, use the same pipette as much as possible and tighten the nozzle.
Why is the ELISA kit dark and all colored, and the critical control OD value is outside the specified range?
1. Insufficient washing, not dry after washing, residual of other components in the sample or residual enzyme labeling - accurate preparation of concentrated washing solution; 10 times concentrated washing liquid should be crystallized, then the crystal should be dissolved at room temperature and then measured. Dilute; wash thoroughly and pat dry thoroughly. The filter paper for adding or adding the enzyme plate should be discarded and not used repeatedly, otherwise it will cause pollution;
2, sample contamination - samples should be freshly collected, or stored at low temperatures to prevent pollution;
3, the incubator temperature exceeds 37 ° C or the reaction time is too long - adjust the incubator temperature, accurate timing;
4, the nozzle is reused, not washed or disinfected incompletely - the nozzle is used as soon as possible;
5. Distilled water is contaminated - using fresh distilled water;
6, the enzyme and other reagents mixed - different batches of reagents should not be mixed;
7. The amount of specimens in one experiment is too much, and the loading time is too long, which leads to the prolongation of the actual reaction time - reasonable arrangement of experiments, avoiding several samples of the enzyme plate at the same time. 
V-Boom's Industrial Co.Ltd , https://www.v-booms.com