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Detection range: 96T20pg/ml-480pg/ml Use purpose: This kit is used to determine the content of P21 (P21) in human serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of human P21 in the specimen. The microporous plate was coated with purified human P21 antibody to prepare a solid phase antibody, and P21 was sequentially added to the microwell of the coated monoclonal antibody, and then bound to the HRP-labeled P21 antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB was added to develop color. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with P21 in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the human P21 concentration in the sample was calculated from a standard curve. Kit composition 1 30 times concentrated washing solution 20ml × 1 bottle 7 Stop solution 6ml × 1 bottle 2 Enzyme standard reagent 6ml × 1 bottle 8 standard product (960pg / ml) 0.5ml × 1 bottle 3 enzyme label coating plate 12 holes × 8 9 standard dilutions 1.5ml × 1 bottle 4 sample dilution 6ml × 1 bottle 10 instructions 1 part 5 color reagent A liquid 6ml × 1 bottle 11 sealing film 2 sheets 6 coloring agent B liquid 6ml × 1 Bottle 12 sealed bag 1
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