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Enzyme-linked immunoassay of rat tumor necrosis factor alpha (TNF-α)
Kit instruction manual
Read this manual carefully before use. This ELISA kit is based on the principle of double antibody sandwich technology to detect rat tumor necrosis factor alpha (TNF-α), which can only be used for research purposes, not for medical diagnosis.
Uses: For the determination of tumor necrosis factor alpha (TNF-α) in rat serum, plasma and related liquid samples.
working principle
This kit uses biotin double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to determine the level of rat tumor necrosis factor alpha (TNF-α) in the sample. Add rat tumor necrosis factor alpha (TNF-α) to the enzyme-labeled wells pre-coated with rat tumor necrosis factor alpha (TNF-α) monoclonal antibody; incubate; after incubation, add biotin-labeled TNF-α antibody. It is combined with streptavidin-HRP to form an immune complex. After incubation and washing, the unbound enzyme is removed, and then substrates A and B are added to produce a blue color, which is converted into the final under the action of acid Yellow. The color depth is positively correlated with the concentration of rat tumor necrosis factor alpha (TNF-α) in the sample.
Assay range
8ng / L → 240 ng / L.
Storage and validity
1. Storage: 2-8 ℃.
2. validity: six months.
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