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Instructions for use Operating room anaerobic environment formation
1) Place the necessary accessories and appliances according to the requirements of use, and put two five-toxic plastic bags into the operating room.
2) Adjust the input pressure of the mixed gas cylinder and nitrogen cylinder, adjust the pressure reducing valve to make the input pressure 0.1Mpa
3) After the device is turned on, after the power switch is turned on, press the main power button on the control card to power on the device.
4) Put 1000g of palladium particles (sealed) and 500g of desiccant in the operation room, and put the Meilan indicator strip.
5) Close the indoor and outdoor doors of the sampling, and vacuum check. (Press the vacuum pump key on the control panel, press once, then press off)
6) The first replacement in the operating room (nitrogen replacement): (1) First insert the rubber tube into the air inlet of the operating room, and the other into the plastic bag. (2) Connect the nitrogen gas inlet, open the nitrogen control valve in the operation room, and make the two plastic bags full of nitrogen, then close the solenoid valve, and then tighten the bag mouth. (3) Put the latex glove on the flange ring of the observation board and tighten it tightly. (4) Gradually put the nitrogen in the plastic bag in the operating room until it is completely eliminated
7) The second replacement of the operation room (nitrogen replacement) repeats the nitrogen flushing process. The sampling room is evacuated first, and pay attention to close the exhaust at any time by pressing the exhaust valve. 8) The third replacement of the operation room (mixed gas replacement) (mixed gas ratio is H2 1/85% H2 1/10% H2 1/10% CO2 / 15%) (1) Change the gas path and open the operation room mixture When the valve is inhaled, the sampling chamber is first evacuated when flushing, and pay attention to close the exhaust by pressing the exhaust valve at any time. (2) After the mixed gas is filled in the plastic bag, close the solenoid valve and open the mixed gas restrictor valve, so that the mixed gas passes through the flow stabilizer, flowmeter, and adjust the flowmeter, and the flow rate is about 10 ml per minute. (3) The mixed gas in the plastic bag is gradually discharged into the operating room. (4) After three times of ventilation, the oxygen content of the gas in the operating room is already in the extreme state
9) Turn on the palladium particle deaerator in the operating room, connect the power of the deaerator catalyst for catalytic deaeration, and drip a little clean water on the reaction area of ​​the anaerobic indicator bar. After 1-2 hours, observe the discoloration and the reaction part Dark blue changes to light blue to achieve an anaerobic environment in the operating room.
Note: 1 The indicator strip can be used repeatedly, 2 Do not touch the blue reaction area 3 Pack the spring in the sealed tube, prevent it from being in a dry place. 10Ultraviolet sterilization lamp, sterilization treatment in the room, the sterilization time is customized.
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