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Recently, scientists from Tsinghua University and the US Master University have jointly developed a new 3D printing method that can produce a highly consistent "module" of embryonic stem cells. These cells can be transformed into all cell types in our body through differentiation, and can be used as "Lego blocks" to construct tissue structures and even micro-organs. Currently, relevant research results have been published in the November 4th issue of Biofabrication.
The first author of this article, the professor of mechanical engineering at Tsinghua University, and the introduction of talents from the National “Thousand Talents Program†Sun Wei explained: “We are very excited to be able to cultivate embryoid bodies in such a controlled way. Growing embryos The morphoids are homogeneous and homogenous and can serve as a better starting point for further tissue growth."
This grid-like three-dimensional structure that can be used to create embryoid bodies is generated by researchers using extrusion-based 3D printing technology that not only displays cell viability and rapid self-renewal in 7 days, but also Maintain high versatility.
Professor Sun explained: "There are two other common methods for printing these cells, namely the two-dimensional method (in one dish) and the "suspension" method (dropping through gravity material, gradually obtaining "stalactite"-like cells). However, the cells printed by these methods did not exhibit the same cell uniformity and homogenous proliferation. I believe that the reason for our successful achievement of higher levels of cell proliferation is to create a three-dimensional microenvironment that is in the human body. The growing environment of the embryoid bodies found is very similar.
Researchers hope that this technology can be further developed to produce embryoid bodies with high throughput, providing a basic building block for other researchers to conduct experimental/drug screening studies on tissue regeneration.
Rui Yao, co-author and assistant researcher in the Department of Mechanical Engineering at Tsinghua University, added: "Our next work is to study how to change the size of the embryo and change the parameters of the embryo and how to "manufacture" Different types of cells.
Professor Sun Wei concluded: "We have always hoped to produce controlled heterogeneous embryoid bodies. This will promote the immediate development of different types of cells and guide the method of preparing micro-organs from scratch in the laboratory. â€
(Editor)
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